FastQCFastQC Report
Thu 6 Aug 2015
G118-M5_GTCCGC-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M5_GTCCGC-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7530261
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG114975615.268474758046235No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT2995623.9781091253012346No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA2701803.58792344647815No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC2091612.777606247645334No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT1751142.3254705248596297No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAATGATAGCACAT842741.11913783599267No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT704230.9351999884200561No Hit
CTTACCATTTCACACAGATCCACACACACACAGCAGATCACCCCCCCACA453210.6018516489667489No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG379990.504617303437424No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC377370.5011380083638536No Hit
CTTACCATTTCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAGC342670.45505726826732834TruSeq Adapter, Index 2 (97% over 34bp)
GAAGCAGTAGCAGAGTGATCTATTTATAATAAGCCCCAGCTTAAGCACTG289770.38480737918645846No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG206090.27368241286722994No Hit
CTTACCATTTCACACAGATCCCCTCTTCTTTACAAAGACAAAGAAGTAGA199280.26463890162638454No Hit
CTTACCATTTCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCCAG189620.251810661011617Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA165390.21963382145718455No Hit
GAAGCAGTAGCAGAGTGATCACATATTGACCTACACAGGAAAGAGAGAAC164790.2188370363258325No Hit
ATATTTCAAGTGGCATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA158730.21078950649917713No Hit
GAAGCAGTAGCAGAGTGATCGTGGTGGCTATCTAGCTTGTCAACTCATCC155350.2063009502592274No Hit
CTTACCATTTCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG150730.20016570474781686Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGCAGTAGCAGAGTGATCGATTGATTGATCGATCGATAGGCACCCAGT147100.19534515470313712No Hit
ATATTTCAAGTGGCATGATCACATTGATTTTTGCATACTCAGTTGCAGAA145820.19364534642291947No Hit
ATATTTCAAGTGGCATGATCCACTTAGATTTGACCTTAGTACTTTTCTAA129830.17241102267238811No Hit
CAATCTTTGAGCTCTTGATCAGGAAAGTACCATTAATTCTCTGCCTTGTG121310.16109667380718942No Hit
ATATTTCAAGTGGCATGATCACCTGCTGTATATACTAGGGGACTCTGGAC118980.15800249154710574No Hit
CACACTGCTCTTAGAGGATCCCGAGACAAAAAGCAAACTCTCCTCTCTGG118670.15759081922924054No Hit
CACACTGCTCTTAGAGGATCATTGCCCAACTAATTTGACCACAAATCCAA104960.13938427897784686No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA104510.13878669012933284No Hit
CATGAGGTTTAGAAAAGATCGGGCTGTGAATCACTGAGTTTTGACCATAG103650.1376446314410616No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA101050.13419189587186953No Hit
CATGAGGTTTAGAAAAGATCGGAAGACGGTGAGCAGAGAATTTAAGATCG98620.13096491608989383No Hit
CTTACCATTTCACACAGATCAAAAGCTACCACCACGCAGACTATCTGAAG93500.12416568296902326No Hit
CACTCTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC87260.11587911760296223No Hit
CAAGCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT84980.11285133410382456No Hit
GAAGCAGTAGCAGAGTGATCCTGCATCTAATGTCCCCCAGCCTCCAAATT84680.11245294153814855No Hit
GAAGCAGTAGCAGAGTGATCTTGATTTAGCAAATGATCTTCATTGATTTT84010.11156319814147211No Hit
CTTACCATTTCACACAGATCAAATGAAAATACTAACTAAGCTACATGAGT82110.10904004522552405No Hit
CACACTGCTCTTAGAGGATCACAAGCATGTACAGATTCTGAGGTAGTTAG76870.10208145507838308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT1059450.0118.2939451
AGTGGCA1517900.0117.598529
GTATGGT1164150.0117.515736
AAGTGGC1522200.0117.446138
GTTTAGA1434150.0117.035557
CAAGTGG1526650.0116.9634867
AGGTTTA1381250.0116.952995
ATGGTTA1173450.0116.797348
TTTGAGC825150.0116.762596
TGAGGTT1360650.0116.713953
GCAGTAG705800.0116.5454564
AAGTATG1081050.0116.535464
GGTTTAG1441150.0116.48366
GTAAGTA1076650.0116.4015352
ATGAGGT1363850.0116.352872
CAGTAGC729250.0116.269735
GAGGTTT1364100.0116.255354
TATGGTT1178250.0116.114487
CATGAGG1367950.0115.946851
TGAGCTC826550.0115.938558
Produced by FastQC (version 0.11.2)