FastQCFastQC Report
Thu 6 Aug 2015
G118-M5_GTCCGC-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M5_GTCCGC-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7530261
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC128864317.112859700347705No Hit
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG123352216.38086647992679No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA101342013.457966463579416No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT6881119.13794355866284No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG3331174.423711210009852No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA3031674.025982631943302No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT2906993.860410681648352No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC1506832.001032899125276No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG1451411.9274365124927275No Hit
TGTTTCAGGTGTATCTGCTGTATGTTTGTATCTGTTGGGTATATGGATCT600830.7978873507837245No Hit
TTTTAAAATCAGCCAGATGGAACCTATTGGGCAGTTTGATCCCTTCCTTT293200.38936233418735416No Hit
TAAATTCTCTGCTCACCGTCTCGGTGCTACCTCCTATCCTGGACTGTGAG238720.3170142442605907No Hit
TTTTAAAATCAGCCAGATGGTCAGGAGGAGCAGGTGGAAGCCAGATGGGA222370.29530184943124815No Hit
TGTTTCAGGTGTATCTGCTGGTGGCTGACACTCCTCTGTCCTCTACTTCT209190.2777991360458821No Hit
TTTTAAAATCAGCCAGATGGCTGACCTATTCTGGAAAGGATTAGAAGTTG199710.26520993097052015No Hit
TTTTAAAATCAGCCAGATGGACTCAGAGAAAAAAACAAAACAAAACAAAA176400.23425482861749414No Hit
TTTTAAAATCAGCCAGATGGTGTGGCCCAGACTTAGGGTGGGTCTTCTGA169620.2252511566332163No Hit
GTGTGAAATGGTAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG157340.20894362094487826Illumina Single End PCR Primer 1 (100% over 35bp)
AAAGAACCTTAGTATCCTGCTGACTGACATCATCGTCCATTGCTTTATCG141170.18747026165494132No Hit
TGTTTCAGGTGTATCTGCTGACCACAGAGAGTCTGCCAATGAGCCTAACT106640.1416152773456325No Hit
TAAATTCTCTGCTCACCGTCTTCCGATCTTTTCTAAACCTCATGAGATCG102620.13627681696557398No Hit
TTTTAAAATCAGCCAGATGGGCCAGGCAGAGACAGACAGCATGAATGTTT92600.12297050527199523No Hit
TTTTAAAATCAGCCAGATGGTTTGATTTGGTCCCCAGAACCTGTGTGTAA83960.11149679938052613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATTC1439400.0118.343411
GAACCTT1528000.0118.2179644
CCTTAGT1530500.0118.176487
AATTCTC1440850.0118.1252063
TTAGTAT1531250.0118.118619
CTTAGTA1530050.0118.075148
CAGTTAC859950.0118.025084
AAGAACC1522650.0117.836182
GGTGTAT567800.0117.690778
GTTACAA868200.0117.5888756
GTCAGTT859350.0117.581272
TCAGTTA861800.0117.578413
TGGTTGA817800.0117.349099
AAATTCT1452850.0117.2642062
AGGTGTA569050.0117.244047
GTCTCCC1160650.0117.237177
TTGTCTC1168600.0117.172785
ATCAGCC731850.0117.114478
ATGGTTG815900.0117.046248
AGTTACA868550.0117.02775
Produced by FastQC (version 0.11.2)