FastQCFastQC Report
Thu 6 Aug 2015
G118-M6_GTGAAA-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M6_GTGAAA-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659172
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG78016913.785921332661385No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT2592424.580917491110007No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA1997403.529491593469857No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC1721703.042317851445406No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT1408462.4888093169813534No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAATGATAGCACAT905061.5992798946559674No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT770831.3620897191320567No Hit
CTTACCATTTCACACAGATCCACACACACACAGCAGATCACCCCCCCACA398040.703353776842266No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC272010.4806533535294562No Hit
GAAGCAGTAGCAGAGTGATCTATTTATAATAAGCCCCAGCTTAAGCACTG249610.44107159139181495No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG237400.41949599694089523No Hit
GAAGCAGTAGCAGAGTGATCACATATTGACCTACACAGGAAAGAGAGAAC183480.3242170409381443No Hit
GAAGCAGTAGCAGAGTGATCGTGGTGGCTATCTAGCTTGTCAACTCATCC158450.2799879558352353No Hit
GAAGCAGTAGCAGAGTGATCGATTGATTGATCGATCGATAGGCACCCAGT149340.26389019453729273No Hit
CTTACCATTTCACACAGATCCCCTCTTCTTTACAAAGACAAAGAAGTAGA147640.2608862215179182No Hit
ATATTTCAAGTGGCATGATCACATTGATTTTTGCATACTCAGTTGCAGAA142560.2519096433188459No Hit
CTTACCATTTCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAGC141100.24932976060808898TruSeq Adapter, Index 2 (97% over 34bp)
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA126710.22390201252055955No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG122180.2158973079454026No Hit
CAATCTTTGAGCTCTTGATCAGGAAAGTACCATTAATTCTCTGCCTTGTG108180.19115870660937678No Hit
CTTACCATTTCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG107550.1900454695492556Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATGAGGTTTAGAAAAGATCGGAAGACGGTGAGCAGAGAATTTAAGATCG105260.18599894118786284No Hit
ATATTTCAAGTGGCATGATCACCTGCTGTATATACTAGGGGACTCTGGAC102750.1815636633769039No Hit
ATATTTCAAGTGGCATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA90870.1605711931003334No Hit
GAAGCAGTAGCAGAGTGATCCTGCATCTAATGTCCCCCAGCCTCCAAATT88000.1554997798264481No Hit
CTTACCATTTCACACAGATCAAAAGCTACCACCACGCAGACTATCTGAAG82260.14535695327867754No Hit
CAAGCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT79180.13991446098475183No Hit
ATATTTCAAGTGGCATGATCCACTTAGATTTGACCTTAGTACTTTTCTAA78330.13841247447506455No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA76080.13443662783177468No Hit
CACACTGCTCTTAGAGGATCCCGAGACAAAAAGCAAACTCTCCTCTCTGG75850.13403020795268283No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA71100.12563675392795978No Hit
CATGAGGTTTAGAAAAGATCGGGCTGTGAATCACTGAGTTTTGACCATAG70120.12390505183443798No Hit
CATGAGGTTTAGAAAAGATCGGAGACGGTGAGCAGAGAATTTAAGATCGG69800.12333959808961453No Hit
GAAGCAGTAGCAGAGTGATCTTGATTTAGCAAATGATCTTCATTGATTTT69570.12293317821052267No Hit
CACTCTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC68410.12088340838553767No Hit
CTTACCATTTCACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCCAG57340.10132224290055154Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAATTTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT56990.10070377786715089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGGC1136550.0117.408378
GGTAAGT619300.0117.405791
CAAGTGG1137000.0117.309587
GGTTTAG991650.0117.12266
AGGTTTA954550.0117.087245
AGTGGCA1138600.0117.0506749
TGAGGTT942750.0117.010813
ATGAGGT941350.0116.9257052
GCAGTAG690450.0116.847834
CAGTAGC711300.0116.794665
GCTCTTA721850.0116.68677
GAGGTTT944100.0116.674384
AGTAGCA729750.0116.670916
GTATGGT681400.0116.628296
GTTTAGA994700.0116.613947
CAATCTT629250.0116.3919751
GAGCTCT692700.0116.3404859
CTCTTAG723850.0116.339628
ATGGTTA684100.0116.333228
GTAGCAG731800.0116.287177
Produced by FastQC (version 0.11.2)