FastQCFastQC Report
Thu 6 Aug 2015
G118-M6_GTGAAA-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M6_GTGAAA-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659172
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC95686816.908268559428834No Hit
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG83077614.680168759670142No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA56870910.04933230514994No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT5562879.829830229581288No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG2880895.090656371638819No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT2352624.157180591082936No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA2200473.8883250058489125No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG1568662.7718895979835922No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC1372072.4245066239372117No Hit
TGTTTCAGGTGTATCTGCTGTATGTTTGTATCTGTTGGGTATATGGATCT535300.9458980925124736No Hit
TTTTAAAATCAGCCAGATGGAACCTATTGGGCAGTTTGATCCCTTCCTTT251260.44398721226356086No Hit
TTTTAAAATCAGCCAGATGGTCAGGAGGAGCAGGTGGAAGCCAGATGGGA230150.40668493553473906No Hit
TTTTAAAATCAGCCAGATGGCTGACCTATTCTGGAAAGGATTAGAAGTTG215380.38058571112523176No Hit
TTTTAAAATCAGCCAGATGGACTCAGAGAAAAAAACAAAACAAAACAAAA178520.31545250789338086No Hit
TTTTAAAATCAGCCAGATGGTGTGGCCCAGACTTAGGGTGGGTCTTCTGA173100.30587513509043374No Hit
TAAATTCTCTGCTCACCGTCTCGGTGCTACCTCCTATCCTGGACTGTGAG170930.3020406518833497No Hit
TGTTTCAGGTGTATCTGCTGGTGGCTGACACTCCTCTGTCCTCTACTTCT152530.2695270615560015No Hit
AAAGAACCTTAGTATCCTGCTGACTGACATCATCGTCCATTGCTTTATCG122430.21633906868354594No Hit
GTGTGAAATGGTAAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG111120.1963538128899422Illumina Single End PCR Primer 1 (100% over 35bp)
TAAATTCTCTGCTCACCGTCTTCCGATCTTTTCTAAACCTCATGAGATCG108680.1920422280856634No Hit
TTTTAAAATCAGCCAGATGGGCCAGGCAGAGACAGACAGCATGAATGTTT94610.16717993374295745No Hit
TGTTTCAGGTGTATCTGCTGACCACAGAGAGTCTGCCAATGAGCCTAACT92200.16292136022725587No Hit
TAAATTCTCTGCTCACCGTCTCCGATCTTTTCTAAACCTCATGAGATCGG72620.12832265921587116No Hit
TTTTAAAATCAGCCAGATGGTTTGATTTGGTCCCCAGAACCTGTGTGTAA69020.12196130458660738No Hit
TTTTAAAATCAGCCAGATGGTTCAAAAGTAGCTATGGACCCGGGGTCTCC65150.11512284836014881No Hit
TTTTAAAATCAGCCAGATGGAAAGCACCTTTACTGCATTATGAATGTTTT58480.10333667186648507No Hit
GGTCAGTTACAATGTGTCCCAGGCTGACCTTGAACTCATTGCATAACAAA57330.1013045724710258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCTT1134700.0118.3764
CCTTAGT1141250.0118.108487
AGGTGTA436300.0118.004467
TTAGTAT1144900.0117.841089
GTTACAA702650.0117.789096
CTTAGTA1143450.0117.777178
TAAATTC988000.0117.759411
AAGAACC1134100.0117.698882
GGTGTAT438750.0117.684568
AATTCTC987400.0117.6441653
ATCAGCC712400.0117.5133368
TCAGTTA699100.0117.468033
AAATTCT991400.0117.259532
GTCAGTT700150.0117.096412
TGGTTGA686750.0117.0246059
AGTTACA705050.0116.966175
ATTCTCT995450.0116.896034
GTGTATC441100.0116.895719
TCAGATG681750.0116.809394
CAGTTAC705450.0116.781784
Produced by FastQC (version 0.11.2)