FastQCFastQC Report
Thu 6 Aug 2015
G118-M7_GTGGCC-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M7_GTGGCC-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7231076
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG85519811.826704628744048No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA4393626.076025200122361No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT1133671.5677749756744364No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC472470.6533882371033025No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT432110.5975735837930621No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT428020.5919174407792146No Hit
ATATTTCAAGTGGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCAGC406440.5620740260508947TruSeq Adapter, Index 2 (97% over 34bp)
CAATCTTTGAGCTCTTGATCCTTTGCTCCCTGGAATGTCAACCATCTGAC382880.5294924296190497No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG270790.37448092095837465No Hit
CATGAGGTTTAGAAAAGATCGGAAGACGGTGAGCAGAGAATTTAAGATCG265130.3666535934624391No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA232080.32094808573440525No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC208250.28799310088844315No Hit
CATGAGGTTTAGAAAAGATCGGAGACGGTGAGCAGAGAATTTAAGATCGG178960.24748737255700257No Hit
CAATCTTTGAGCTCTTGATCCTTTGCTCCCTGGAATGTCCACCATCTGAC171670.23740588537584173No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA146550.20266693366243144No Hit
ATATTTCAAGTGGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACG143930.1990436831254436Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG141080.19510236097643005No Hit
ATATTTCAAGTGGCATGATCAGCAGGATACTAAGGTTCTTTAGATCGGAA136120.18824307751709426No Hit
ATATTTCAAGTGGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCCAG135510.18739949628520017Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA128600.17784351872390777No Hit
CTTACCATTTCACACAGATCCCCTCTTCTTTACAAAGACAAAGAAGTAGA105500.14589806551611406No Hit
CACACTGCTCTTAGAGGATCCACCCATTTGTACAGATTCTGAGGTAGTTA96710.13374219825652503No Hit
ATATTTCAAGTGGCATGATCCAGCAGGATACTAAGGTTCTTTAGATCGGA94380.13051999453469995No Hit
GGTAAGTATGGTTAATGATCTGTGAGCACGGAGACTGTGTCAAGAATTAA88160.12191823180948451No Hit
CACACTGCTCTTAGAGGATCTCATGGAGGCATTTCCTCAAGGGAGGCTCC85490.11822583526988238No Hit
CACACTGCTCTTAGAGGATCATTCCCAGCCTCCCAGAGGGTCACCATAGT84410.1167322816134141No Hit
CACACTGCTCTTAGAGGATCTTAGCGGTACAGATTCTGAGGTAGTTAGCA77320.10692737844271033No Hit
CTTACCATTTCACACAGATCCATCTTGCCTACTGAACCTTTTCCTTTAGC77070.10658164842963896No Hit
GGTAAGTATGGTTAATGATCAGTCCAAACTTCTTTCTGCATGTACATAAA73140.10114677262415717No Hit
CATGAGGTTTAGAAAAGATCGGGCTGTGAATCACTGAGTTTTGACCATAG72680.10051062940010587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAGC917600.0116.936026
GTTTAGA1249300.0116.89777
CTCTTAG1152000.0116.5395368
GGTAAGT763650.0116.489871
AAGTGGC1265600.0116.4217158
CATGAGG1195400.0116.3798451
TGAGGTT1196450.0116.283863
GTATGGT835400.0116.2060856
GAGGTTT1196350.0116.131084
AGTGGCA1269450.0116.0920649
CAAGTGG1268450.0116.07577
AGGTTTA1214600.0116.0275
GCAGTAG700250.0115.931924
GTAGCAG741550.0115.860767
ATGAGGT1199100.0115.853212
AGTAGCA742200.0115.839476
CAATCTT828800.0115.837561
TTACCAT833400.0115.7693562
GGTTTAG1264450.0115.647676
GCTCTTA1162400.0115.573647
Produced by FastQC (version 0.11.2)