FastQCFastQC Report
Thu 6 Aug 2015
G118-M7_GTGGCC-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M7_GTGGCC-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7231076
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG100695013.925313466488252No Hit
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC88918912.296772983716393No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT88225012.200812161288306No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA72902410.081819081973416No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA6308758.724496879855778No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT3115174.308031059278038No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC2454233.394003879920499No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG1252651.7323148034953582No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG463610.6411355654400535No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGGATCAAGAGCTCAAAGAT445160.615620690475387No Hit
TAAATTCTCTGCTCACCGTCTTCCGATCTTTTCTAAACCTCATGAGATCG276790.3827784412720873No Hit
TAAATTCTCTGCTCACCGTCTCGGTGCTACCTCCTATCCTGGACTGTGAG195990.27103850104742366No Hit
TAAATTCTCTGCTCACCGTCTCCGATCTTTTCTAAACCTCATGAGATCGG187050.2586751957799918No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGGATCAAGAGCTTAAAGAT183690.25402858440431275No Hit
AAAGAACCTTAGTATCCTGCTGACTGGAGTTCAGACGTGTGCTCTTCCGA183030.25311585716980434TruSeq Adapter, Index 2 (96% over 32bp)
AAAGAACCTTAGTATCCTGCTGATCATGCCACTTGAAATATAGATCGGAA159710.22086616155050787No Hit
AAAGAACCTTAGTATCCTGCTGGATCATGCCACTTGAAATATAGATCGGA113180.15651889151766624No Hit
TGTTTCAGGTGTATCTGCTGGTGGCTGACACTCCTCTGTCCTCTACTTCT109610.15158186693100723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAC1132200.0118.090864
GGTGTAT836000.0117.911078
AGGTGTA835000.0117.895527
TCAGTTA1133600.0117.808543
TAAATTC1258150.0117.775151
GGTCAGT1127150.0117.701421
GTCAGTT1132500.0117.6497652
TTCAGGT839300.0117.6034244
GTGTATC836900.0117.563879
AGTTACA1139250.0117.4749765
GTTACAA1140500.0117.43496
AATTCTC1262150.0117.3311843
TTACAAT1147650.0117.055827
AAATTCT1267250.0116.873082
TCAGGTG843000.0116.8472755
CCTTAGT1250850.0116.8023767
ATCAGCC725500.0116.802088
ACAATGT1145200.0116.781499
GTCTCCC827100.0116.726777
TTTCAGG851000.0116.566883
Produced by FastQC (version 0.11.2)