FastQCFastQC Report
Thu 6 Aug 2015
G118-M8_GTTTCG-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M8_GTTTCG-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997696
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG135464710.422208674522007No Hit
CTTACCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA7023845.40391158556101No Hit
CAATCTTTGAGCTCTTGATCGATCTTCAATTAGGTCCTCATGCAGGAGTT2059151.5842423149456644No Hit
CACACTGCTCTTAGAGGATCCTAACTTGGTTCCTAGCACCCAAACTGGGC1122490.8636069038697319No Hit
CAATCTTTGAGCTCTTGATCCTTTGCTCCCTGGAATGTCAACCATCTGAC885840.6815361737957251No Hit
CAATCTTTGAGCTCTTGATCCATCTTCAGTTAGGTCCTCATGCAGGAGTT735900.5661772671094939No Hit
GAAGCAGTAGCAGAGTGATCTTCACAAAACCAAAATGAACGATAGCACAT559800.43069171643959053No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA539050.4147273486008597No Hit
CATGAGGTTTAGAAAAGATCGGAAGACGGTGAGCAGAGAATTTAAGATCG509940.3923310715991511No Hit
CATGTGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG382780.2944983480149097No Hit
CATGAGGTTTAGAAAAGATCGGAGACGGTGAGCAGAGAATTTAAGATCGG342600.2635851769421288No Hit
CAATCTTTGAGCTCTTGATCCTTTGCTCCCTGGAATGTCCACCATCTGAC320560.24662832551246003No Hit
GAAGCAGTAGCAGAGTGATCTCAGAACTAAGTTGTGAGGGGCTGAAGCAC307990.23695738075425063No Hit
CTTTCCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA224750.17291526128938545No Hit
CATTAGGTTTAGAAAAGATCTGGCTGTGAATCACTGAGTTTTGACCATAG202030.15543524021488117No Hit
CTTATCATTTCACACAGATCTGTTTTTACCCTGTCTCAGTCTCAGAAAGA201210.1548043591725795No Hit
ATATTTCAAGTGGCATGATCCTCCGGTAGTACTTTTCTAAGTATATTTGT190890.1468644904450758No Hit
ATATTTCAAGTGGCATGATCGGAAGAGCACACGTCTGAACTCCAGTCAGC172620.13280815307574512TruSeq Adapter, Index 2 (97% over 34bp)
ATATTTCAAGTGGCATGATCTCCTTTGGAATCTGCCCTTGGGCCCTTGCT163630.1258915426241697No Hit
CACACTGCTCTTAGAGGATCCTCCGGTAGTACAGATTCTGAGGTAGTTAG160570.12353727922240988No Hit
ATATTTCAAGTGGCATGATCTGCAACCCTATAGGTGGAACAACATTATGA144830.11142744067871721No Hit
CACACTGCTCTTAGAGGATCTTTGTGGCATCTTCCTAAGCCTCTGACCTC142760.10983485073046792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGGC2562750.0117.23768
GTATGGT1699800.0117.216956
CAAGTGG2562500.0117.181717
GGTAAGT1568750.0116.95441
AGTGGCA2572250.0116.811559
GTTTAGA2001650.0116.522027
TGAGGTT1916650.0116.4748153
AGGTTTA1941450.0116.390115
TATGGTT1710650.0116.351747
CTCTTAG2209750.0116.2325748
ACACTGC2103500.0116.211682
GAGGTTT1917200.0116.150594
ATGGTTA1720650.0116.024788
CATGAGG1924250.0115.885131
GCAGTAG1055000.0115.719234
ATGAGGT1928100.0115.619592
GAGCTCT1731400.0115.5758749
GGTTTAG2021200.0115.5303656
TTTGAGC1739400.0115.530036
TTGAGCT1737000.0115.453317
Produced by FastQC (version 0.11.2)