FastQCFastQC Report
Thu 6 Aug 2015
G118-M8_GTTTCG-_AC6PMVANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M8_GTTTCG-_AC6PMVANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12997696
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACCTTAGTATCCTGCTGTTTTATAGGTTATCTATCCTAAACTTCC211402416.264605665496408No Hit
GGTCAGTTACAATGTGTCCCAGTGCTGTTGCAAAATATGTCTTTGTTCTT166833812.835644101846974No Hit
TAAATTCTCTGCTCACCGTCCCGGTGCTACCTCCTATCCTGGACTGTGAG158518112.195861481911871No Hit
CTCTTTGTCTCCCATTTGAGCATTTCAGTGTGGGCCTTGGCATGGAAGCA146453611.267658514247449No Hit
TGTTTCAGGTGTATCTGCTGAGTCATTACCGCAGCTCCCATGAGAAGATA9026136.944407685792927No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGCACTGCTCACCCTATTGT5441334.186380416960052No Hit
TTTTAAAATCAGCCAGATGGCAAAGCCTCTCAGCATGGAATCTATGTTCC3530772.7164583630821957No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGTACAGTCCAGTATACTGG2277101.7519258797866946No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGGATCAAGAGCTCAAAGAT1026030.7893937510155645No Hit
TTTTAAAATCAGCCAGATGGGACAGGACATCCACACTGAGTCACATGTGG604330.4649516345050692No Hit
TAAATTCTCTGCTCACCGTCTTCCGATCTTTTCTAAACCTCATGAGATCG528090.40629508491351085No Hit
TAAATTCTCTGCTCACCGTCTCCGATCTTTTCTAAACCTCATGAGATCGG356250.274087038195077No Hit
AGGTCAGATGGTTGACATTCCAGGGAGCAAAGGATCAAGAGCTTAAAGAT337870.25994607044202295No Hit
TAAATTCTCTGCTCACCGTCTCGGTGCTACCTCCTATCCTGGACTGTGAG298450.2296176183840582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAC2149600.0117.906674
CCTTAGT2568000.0117.822987
GTTACAA2163650.0117.685526
CTTAGTA2571250.0117.583818
GGTCAGT2136600.0117.565831
TCAGTTA2155150.0117.553333
TTAGTAT2576450.0117.5520259
GTCAGTT2149800.0117.530362
AAGAACC2554650.0117.530352
GGTGTAT1188750.0117.423348
GTGTATC1186450.0117.294929
AATTCTC2003350.0117.289273
TAAATTC2007750.0117.248561
AGTTACA2166800.0117.195895
GAACCTT2576500.0117.175634
TGGTTGA1704950.0117.164359
TTCAGGT1199950.0117.1455154
ATTCTCT2013200.0116.996184
TTACAAT2183000.0116.942177
AGAACCT2574750.0116.811583
Produced by FastQC (version 0.11.2)