FastQCFastQC Report
Thu 6 Aug 2015
G118-M9_CGTACG-_AC6PMVANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG118-M9_CGTACG-_AC6PMVANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906474
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAAGTATGGTTAATGATCCAAGAAAGAATTATCTCTTCTGTTTAGTTT383950.7825375208347175No Hit
GGTAAGTATGGTTAATGATCTGTGAGCACGGAGACTGTGTCAAGAATTAA307220.6261523040782444No Hit
GGTAAGTATGGTTAATGGGAGACAAAGAGAGATCGGAAGAGCACACGTCT269540.5493558103028774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTAAGTATGGTTAATGATCAATGTTCTATCCCAGACCCATCAATTGTGA259640.529178387575273No Hit
GGTAAGTATGGTTAATGATCTACAGTTATTGGTTAAAGAAGTATATTAGA211840.43175608390057707No Hit
GGTAAGTATGGTTAATGATCAATTTCATTTTAGTCATCAACATTACATCA206770.42142279771583424No Hit
GGTAAGTATGGTTAATGATCACAGGGCTCCCAATGGAGGAGCTAGAGAAA192590.39252220637467966No Hit
GGTAAGTATGGTTAATGATCCTGAGTTTCAACTGGTAAAGAGCCATAGTC184350.37572806867008773No Hit
GGTAAGTATGGTTAATGATCACAGGGCCCCCAATGGAGGAGCTAGAGAAA167200.34077425051065185No Hit
GGTAAGTATGGTTAATGATCAACTTTTCCAGAATCCACACACAAGCAGGT158560.3231648634029244No Hit
GGTAAGTATGGTTAATGATCACTCTTTCCACCCCATCTCCGGAGCCAGGA150890.30753245609780056No Hit
GGTAAGTATGGTTAATGATCCAGCAATACCTCTCCTGGGCATATATCCAG140200.2857449157990035No Hit
GGTAAGTATGGTTAATGATCAAAGAATAATATATAAAACTATTTAAATCT134930.2750040049126929No Hit
GGTAAGTATGGTTAATGATCGCATAGGTAGCTAAAGAGGTGGCAACCCAC120930.24647027580294933No Hit
GGTAAGTATGGTTAATGATCCTTAACCCCATGTATCCTCTCCTACCTTTG120440.2454715952841083No Hit
GGTAAGTATGGTTAATGATCAATTCCTCTTTAAACTTCCTAGAAAGAGTA120180.2449416831720702No Hit
GGTAAGTATGGTTAATGATCAACTAGGCCATCCTCTGCTCCATATGCAGC115890.23619813332344167No Hit
GGTAAGTATGGTTAATGATCTTTTTACTGTCATTGTATAGTCTGTGGTAT115460.23532174021507093No Hit
GGTAAGTATGGTTAATGATCACAGGGCCCCCAATGGAGGAGCTTGAGAAA105050.21410487449846877No Hit
GGTAAGTATGGTTAATGATCATAGGGCTCCCAATGGAGGAGCTAGAGAAA104280.21253551939743287No Hit
GGTAAGTATGGTTAATGATCATCTTATGGCCAGATTCAGGAGGCTTGGCT98500.2007551655221244No Hit
GGTAAGTATGGTTAATGATCTTGACTCAGAACAATATATGTGTGCACACG98430.2006124968765757No Hit
GGTAAGTATGGTTAATGATCTCATAGAAAAAAAGTCAAGGTGGATAGATA83680.17055017513595302No Hit
GGTAAGTATGGTTAATGATCCCATAGAGTAGAACGGTGTTTACAGGAGAC83140.16944958844172006No Hit
GGTAAGTATGGTTAATGATCAATACAGTCCTCTGCCTTTAAAGCAATAGG80650.1643746609072014No Hit
GGTAAGTATGGTTAATGATCACATATGGAATAGTCATAATCGAACCCATT79110.1612359507051296No Hit
GGTAAGTATGGTTAATGATCATTAGATACAGATTCAAAACCATAATGTGT76800.1565278854020219No Hit
GGTAAGTATGGTTAATGATCTCAAAGCACTGTTATTAACTTGGTTGCAGG76190.1552846300622402No Hit
GGTAAGTATGGTTAATGATCACTTCCAGTCATAGTCAAGATCTTAATGTG75750.15438785571879113No Hit
GGTAAGTATGGTTAATGATCTAGCATAAAAAAAATCTTCCATAATTTTAT74510.151860582569071No Hit
GGTAAGTATGGTTAATGATCCACTACAGCACTTGGTAACATGCTCACTCA73830.1504746585837406No Hit
GGTAAGTATGGTTAATGATCAGTCCAAACTTCTTTCTGCATGTACATAAA73070.14892568471778306No Hit
GGTAAGTATGGTTAATGATCCACAAAATCAGAAGGTACATAAAAGCATAT70760.1442176194146754No Hit
GGTAAGTATGGTTAATGATCTTCCTGCCTTAGTCTCCCAAGTAGTTGCAA67850.13828668000686442No Hit
GGTAAGTATGGTTAATGATCTCATTTTCTCCAACATTGTCCTATTATCTC66860.13626893773410395No Hit
GGTAAGTATGGTTAATGATCCTCCGGTAGTACATAAAAGCATATTTGGGA64920.13231497812889662No Hit
GGTAAGTATGGTTAATGATCAAAATCTTAGGCTGGAATTCAAAGCAGGTG64510.13147934749068274No Hit
GGTAAGTATGGTTAATGATCACTTCTAAAACTTTAGAATTTATTGTTTTA63130.12866673704986514No Hit
GGTAAGTATGGTTAATGATCATGTCCATTCATAGGCATGTAGAACGCAGC57630.1174570577567516No Hit
GGTAAGTATGGTTAATGATCCAAAGGTATATGCTATAGTTTAAAAATTAT56920.11600999006618601No Hit
GGTAAGTATGGTTAATGATCATGTGCTGCTATGGCAACTTCCTGGTCATG55590.11329928580076037No Hit
GGTAAGTATGGTTAATGATCCATCTGAAGCTTGGGTGTGGAAAGAACAGT55420.11295280480442778No Hit
GGTAAGTATGGTTAATGATCAACTTAGAGCTTGGTTCTGAACAAGACCTT54490.11105734994213767No Hit
GGTAAGTATGGTTAATGATCATGTTAAATATACATGTGTATATGCGTATG52780.10757215874373328No Hit
GGTAAGTATGGTTAATGATCAACATTGTAAAAATGGCTATCTTGCCAAAA52370.10673652810551937No Hit
GGTAAGTATGGTTAATGATCTGGCTGTAATCATTTGGAGAGTGGGAATGA52150.10628814093379482No Hit
GGTAAGTATGGTTAATGATCTCACTTAAGTCCATGACTTTAAGCACTAAT52150.10628814093379482No Hit
GGTAAGTATGGTTAATGATCACAAGGCTATCAAGCACTTTGCACATGGCA51920.10581937252699188No Hit
GGTAAGTATGGTTAATGATCAACAAAACGTTGAGGGTAAGTCACAGTCAT50730.10339400555266368No Hit
GGTAAGTATGGTTAATGATCCCTGGATATGGCAGTCTCTACATGGTCCAT49430.10074444499247322No Hit
GGTAAGTATGGTTAATGATCACTTGAGCTATTTTCTCTTTATTCAGAAAC49130.10013300794012156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT4337200.0118.650111
AGTATGG4557650.0118.4540945
GTATGGT4723500.0118.44536
ATGGTTA4733550.0118.368538
TATGGTT4730550.0118.275077
TAAGTAT4350150.0118.259523
AAGTATG4375950.0118.153234
GTAAGTA4374600.0117.6502462
TGGTTAA4781950.0117.192889
GGTTAGT207300.0110.766881
TAGTATG220900.0105.660044
TGTATGG198600.0105.153785
TTAGTAT224750.0102.258613
GGTATGT174450.0101.733481
GTTAGTA244700.093.9459462
GTACGGT3600.092.552256
GGTACGT6050.087.668751
AGTACGG3550.085.474955
GTATGTA222450.079.780322
TACGTAT6050.079.653443
Produced by FastQC (version 0.11.2)