FastQCFastQC Report
Thu 6 Aug 2015
G119-M12_GAGTGG-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M12_GAGTGG-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18364863
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG325440.17720796501449534Illumina Single End PCR Primer 1 (100% over 50bp)
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG271000.14756440056209513No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG199450.10860413170520249No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT73150.048.804137
TCTCGAC24000.031.4856978
TAGCGTC64450.029.35758476-77
ACTCTCG29750.028.20016
GTAGCGA33400.027.7906658
GCCGAGA100400.027.6760438
TATACGC55650.027.63831110-111
TACGCTA54750.027.168896112-113
AAACCCG66400.026.479239108-109
AGTAGCG35200.026.3694827
CGGTAGT36450.025.3017333
TCTAACG88700.025.2222547
CGCTATT57150.025.142994114-115
CTCTCGA32000.024.729847
CGGCGTA68700.024.59674872-73
TACTCGT79000.024.36482662-63
ACGAGCT76400.024.220582-83
ATCGGTA38850.024.0449981
GCGTAGC69250.023.97179474-75
ACTCGTC72500.023.79995264-65
Produced by FastQC (version 0.11.2)