FastQCFastQC Report
Thu 6 Aug 2015
G119-M13_ACTGAT-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M13_ACTGAT-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16850594
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG937700.5564788992008235No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG592580.3516671281736418No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG512370.3040664323168667No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC363110.21548795253152497No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC355630.2110489398771343No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC325580.19321574064392033No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC264770.15712799204585903No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT260530.15461176027385148No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT248960.14774553348089686No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT237530.1409623898124897No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA227590.13506348796962292No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT222110.13181137709448107TruSeq Adapter, Index 25 (97% over 44bp)
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC192320.11413247509256945No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA185500.11008514002533085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT147200.080.63247
ACTCTCG35950.047.8270386
CTCGACT36800.045.5906529
TCTCGAC38750.043.296418
GGCCTCC304050.039.5062529
TAACACG74200.035.6450926-27
CGGCCTC346500.034.5804568
AAACCCG181750.034.219646108-109
CTCTCGA50550.033.8957987
TGCGGCG355950.033.762683
TAGCGTC192600.033.70101576-77
TTCGTGC21950.033.3384259
GTAGCGA107450.033.1661348
AGTAGCG109450.032.66887
TAGCGAC112050.032.282439
CCTGCGG375500.032.2225531
CGGGTCT112800.031.90960574-75
CGGTAGT113750.031.6953623
AGGGGGC256000.031.1602231
CGTAGCG208500.030.98838274-75
Produced by FastQC (version 0.11.2)