FastQCFastQC Report
Thu 6 Aug 2015
G119-M13_ACTGAT-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M13_ACTGAT-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16850594
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG268760.1594958610954605No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG211270.1253783694509523No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG202730.1203102988535597Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT73400.048.151327
GCCGAGA89350.038.6234668
TATACGC56000.032.937466110-111
GTAGCGA30500.032.578758
TACGCTA55850.032.439983112-113
TCTCGAC20050.031.753188
AGTAGCG31800.031.6211327
TAGCGTC58250.031.2055576-77
CGCTATT58350.030.132362114-115
AAACCCG61500.029.024359108-109
CGGTAGT36150.028.3099613
ATCGGTA36800.028.1332931
ACGAGCT73650.026.86181882-83
CGGCGTA64050.026.70762372-73
TACGAGC64550.026.1781382-83
ACGCTAT57300.026.115591114-115
CTACGAG77300.025.8243680-81
ATATACG60400.025.711067110-111
ACTCTCG25700.025.6984946
AACTACG77900.025.66364578-79
Produced by FastQC (version 0.11.2)