FastQCFastQC Report
Thu 6 Aug 2015
G119-M14_ATTCCT-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M14_ATTCCT-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19544546
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1135750.5811084074298783No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG654190.33471741937622906No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG386980.19799897117078083No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC373000.19084608053827395No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC351120.17965114155120307No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT332880.17031861471737433TruSeq Adapter, Index 27 (97% over 44bp)
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA331880.16980696302692322No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC316450.1619121774432622No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC293470.15015442159669506No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT246240.12598911225668788No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT206030.10541559778364767No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC205500.10514442238770856No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA196650.10061630492721602No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT170750.081.601917
CTCGACT46050.046.8976559
TCTCGAC46650.046.294478
ACTCTCG47400.045.687486
GGCCTCC357200.039.7404829
CGGCCTC366200.038.27648
AGTAGCG115150.035.804987
GTAGCGA116350.035.43578
CTCTCGA63250.034.4266477
CGGTAGT124000.034.017213
TGCGGCG415350.033.961863
TAGCGAC126250.033.599469
TAGCGTC228400.032.5993976-77
AAACCCG209900.032.280914108-109
TCGGTAG132850.031.5719742
CCTGCGG453900.031.4719351
AGGGGGC277200.031.0319021
ATCGGTA142200.029.5341191
GCCGAGA286250.029.1391358
CGTAGCG256700.029.08668574-75
Produced by FastQC (version 0.11.2)