FastQCFastQC Report
Thu 6 Aug 2015
G119-M4_AGTTCC-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M4_AGTTCC-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21713562
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT3516221.6193658138632434TruSeq Adapter, Index 14 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG478500.048.90320248-49
CGTATGC495450.047.47661646-47
TCGTATG498600.047.3366244-45
GCCGTCT499600.046.87881550-51
CTCGTAT495100.046.59573744-45
ATGCCGT503200.046.52639848-49
TGCCGTC504550.046.277450-51
CCGTCTT513950.045.28024752-53
TATCTCG501550.043.57119840-41
TCTCGTA507200.043.50276642-43
CGTCTTC541050.043.08923752-53
ATCTCGT513700.042.65117642-43
GTATGCC558350.042.3679746-47
CGTATCT520950.041.84007638-39
CCGTATC525800.041.2617838-39
TCCGTAT545400.040.3512536-37
GCTTGAA578450.039.8800660-61
TTCCGTA589200.039.2045636-37
TGCTTGA592800.038.9397760-61
GTTCCGT606200.038.47436534-35
Produced by FastQC (version 0.11.2)