FastQCFastQC Report
Thu 6 Aug 2015
G119-M5_ATGTCA-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M5_ATGTCA-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850804
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10960505.256631830599914Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC2847300.056.7299049
CGTATCA1227350.055.3646848-49
AGAGCGT2940300.054.9979028
CCGTATC1234250.054.97562848-49
CGCCGTA1230550.054.65498746-47
GCCGTAT1251650.054.5488946-47
TCGCCGT1234350.054.11315544-45
GTCGCCG1321200.053.44271544-45
GTATCAT1271600.053.1198850-51
AAGAGCG3043300.052.999657
GGTCGCC1334600.052.7523142-43
TGGTCGC1337100.052.33551442-43
GTGGTCG1365000.052.00028640-41
ATCATTA1352850.051.87135352-53
CGGTGGT1448850.050.26186838-39
TCGGTGG1511600.049.86598638-39
CATTAAA1439500.049.79472754-55
TCTCGGT1488650.049.75144236-37
TCATTAA1425800.049.62844552-53
ATCTCGG1506500.049.60627734-35
Produced by FastQC (version 0.11.2)