FastQCFastQC Report
Thu 6 Aug 2015
G119-M7_GTCCGC-_AC6PMVANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M7_GTCCGC-_AC6PMVANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22314056
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT6300812.823695521782324TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG800200.053.51184548-49
TCGTATG819000.052.65197844-45
CGTATGC818500.052.6063146-47
CTCGTAT804800.052.29837844-45
ATGCCGT823650.051.8980248-49
GCCGTCT821300.051.8538550-51
TGCCGTC824350.051.79551750-51
CCGTCTT824350.051.340352-53
TCTCGTA797800.049.92829542-43
GTATGCC864250.049.76646846-47
ATCTCGT803550.049.39331442-43
CGTCTTC862300.049.1291152-53
CGCACAT844150.047.65791336-37
CATCTCG836500.047.48628240-41
GCTTGAA888150.047.10401560-61
TGCTTGA903600.046.1702160-61
TCCGCAC937050.045.8898334-35
CGTCCGC943700.045.7971732-33
GTCCGCA943850.045.56867634-35
CCGCACA938300.045.51043336-37
Produced by FastQC (version 0.11.2)