FastQCFastQC Report
Thu 6 Aug 2015
G119-M9_GTGGCC-_AC6PMVANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M9_GTGGCC-_AC6PMVANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18927529
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG388730.20537810297371623No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT282130.1490580201990445No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC273070.14427134149418025No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG272830.1441445420582898No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG243350.1285693446830804No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC232170.12266260429451725No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA229240.12111459451468809No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG219100.11575731834831689No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG217220.11476405610050841No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC207960.10987171119906883No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG207450.10960226239780163Illumina Single End PCR Primer 1 (100% over 50bp)
AGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGG204410.10799613620985603No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA193160.10205241265249151No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC192830.10187806342814217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAC31150.056.158218
ACTCTCG34050.052.2485966
CTCTCGA36400.048.548867
GCGGCCT59800.047.0634657
AGTAGCG37300.044.346567
GTAGCGA38350.042.9772268
CGGTAGT42550.039.4338263
CTCGACT47900.036.6448339
ATCGGTA45450.036.5249561
GCATAGT101700.036.273562
GGCATAG107350.034.419851
CTCGATC63550.033.98654630-31
AGATCGT55850.033.2397469
GCCAGTA158600.032.7140164
TAAGCCG50500.032.5189931
CCAGTAG159050.032.434495
TAGCATA164300.032.1227239
GTAGCAT165050.031.8684338
TCGGTAG54300.031.3390332
CCGTACG65400.031.25107282-83
Produced by FastQC (version 0.11.2)