FastQCFastQC Report
Sun 2 Apr 2017
G121-M11_GGCTAC-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M11_GGCTAC-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25036570
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1059820.42330878391089516TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA134600.041.5527421
ATCGGAA137850.040.3940852
TCGTATG136600.040.23988743
CTCGTAT133700.040.12912442
ACGGCTA134050.040.00455532
CGTATGC137450.039.94241344
TCTCGTA126000.039.78923441
CGGCTAC136850.039.08558333
ACACGTC143550.039.00936513
CGTCTGA145200.038.61161416
ATCTCGT131000.038.25377340
TCACGGC144450.037.50645430
AGTCACG146200.037.43529528
ACGTCTG150700.037.2899615
GTCACGG146000.037.21451629
TCGGAAG151850.036.7105143
CATCTCG134900.036.6761739
CACGGCT150250.035.86731
CGGAAGA155550.035.8278124
CACGTCT157850.035.65662414
Produced by FastQC (version 0.11.2)