FastQCFastQC Report
Sun 2 Apr 2017
G121-M7_CAGATC-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M7_CAGATC-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24795774
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1817040.7328022912291425TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA230000.041.9852641
CGTATGC228400.041.8164644
ATCGGAA232050.041.6280062
TCGTATG229700.041.59986543
CTCGTAT224550.041.4807342
TCTCGTA213350.041.28780441
CGTCTGA239200.040.823916
ACACGTC240750.040.56065813
ATCTCGT217550.040.480640
ACGTCTG244400.040.05425615
CATCTCG220300.039.6171539
TCGGAAG245950.039.375723
CACGTCT249750.039.17830314
CGGAAGA249900.038.740274
CACACGT258550.037.819312
GCACACG257950.037.77794611
GATCATC238350.036.33113536
ACCAGAT277550.034.00471532
GAACTCC290250.033.59956421
AGATCAT267500.033.1623735
Produced by FastQC (version 0.11.2)