FastQCFastQC Report
Sun 2 Apr 2017
G121_M7-M12.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M7-M12.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156113455
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8440220.540646544527504Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT1320350.042.18322411
CGTCGTG1325000.042.16643512
AGCGTCG1324850.041.97683710
GATCGGA1334150.041.8981361
ATCGGAA1345750.041.498892
TCGTGTA1357850.041.10845614
GTCGTGT1370000.040.93473413
CGTGTAG1370800.040.76985515
GAGCGTC1378650.040.3290829
GATCTCG1148850.040.19331434
TGGTCGC1037000.040.12720542
AGAGCGT1399250.039.8938758
GTGGTCG1077350.039.8259841
ATCTCGG1183500.039.44177235
TCGGAAG1423700.039.3254853
GTCGCCG1076450.039.16760344
AAGAGCG1420250.039.1372077
CGGAAGA1443750.038.4837154
TCTCGGT1195600.038.33959636
GGTCGCC1101500.038.24085243
Produced by FastQC (version 0.11.2)