FastQCFastQC Report
Fri 21 Apr 2017
G116-M1_ATCACG_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M1_ATCACG_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19849169
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1255250.6323942327258134Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG147150.043.1993844
AGCGTCG153350.042.9994610
CGTCGTG154700.042.8801412
GCGTCGT154100.042.77590611
GATCGGA157650.042.307681
TGGTCGC150150.042.13353742
GGTCGCC151300.041.94259643
GTGGTCG152750.041.8636941
GAGCGTC158050.041.734689
ATCGGAA159650.041.558642
GATCTCG156800.041.5292234
GTCGTGT159850.041.51260413
TCGTGTA158800.041.289214
TCGGTGG158900.040.9526738
CGTGTAG160900.040.94199815
AGAGCGT162400.040.846928
TCTCGGT159050.040.83103636
TCGGAAG163500.040.6359943
CGGTGGT158850.040.5083939
AAGAGCG163450.040.422747
Produced by FastQC (version 0.11.2)