Basic Statistics
Measure | Value |
---|---|
Filename | G116-M2_CGATGT_BC72YLANXX_L008_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 26637024 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 565117 | 2.1215470617138013 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCGTCG | 65790 | 0.0 | 43.660667 | 10 |
GCGTCGT | 65895 | 0.0 | 43.641163 | 11 |
CGTCGTG | 66065 | 0.0 | 43.632236 | 12 |
GTCGCCG | 64400 | 0.0 | 43.62129 | 44 |
GATCGGA | 66715 | 0.0 | 43.55293 | 1 |
GAGCGTC | 66165 | 0.0 | 43.3567 | 9 |
ATCGGAA | 66985 | 0.0 | 43.27739 | 2 |
TGGTCGC | 64835 | 0.0 | 43.18981 | 42 |
GTCGTGT | 67100 | 0.0 | 43.175472 | 13 |
GGTCGCC | 65035 | 0.0 | 43.10935 | 43 |
GTGGTCG | 65485 | 0.0 | 43.081158 | 41 |
TCGTGTA | 66640 | 0.0 | 43.028667 | 14 |
TCGGAAG | 67600 | 0.0 | 42.97923 | 3 |
GATCTCG | 66535 | 0.0 | 42.9688 | 34 |
AGAGCGT | 67230 | 0.0 | 42.94788 | 8 |
CGTGTAG | 67140 | 0.0 | 42.92173 | 15 |
AAGAGCG | 67335 | 0.0 | 42.87389 | 7 |
TCGGTGG | 67325 | 0.0 | 42.817623 | 38 |
CGGTGGT | 66845 | 0.0 | 42.736603 | 39 |
CGGAAGA | 67395 | 0.0 | 42.722363 | 4 |