FastQCFastQC Report
Fri 21 Apr 2017
G116-M4_TGACCA_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M4_TGACCA_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31533368
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15967925.063816843161187Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1893300.043.7466771
AGCGTCG1870150.043.71397410
GTCGCCG1806700.043.71193744
CGTCGTG1878200.043.70813412
GCGTCGT1874000.043.6910711
ATCGGAA1889200.043.6862
GAGCGTC1873450.043.5853239
GTCGTGT1890150.043.54213713
TCGGAAG1900600.043.5275843
TCGTGTA1873900.043.49511314
CGGAAGA1888350.043.3834274
AGAGCGT1887550.043.3737958
TGGTCGC1819750.043.3669542
AAGAGCG1888150.043.3293847
GGTCGCC1820650.043.31989343
GTGGTCG1834500.043.31163441
CGTGTAG1891700.043.3111315
GATCTCG1862250.043.2677434
TCGGTGG1873700.043.193638
TCTCGGT1862850.043.1911936
Produced by FastQC (version 0.11.2)