FastQCFastQC Report
Fri 21 Apr 2017
G116-M5_ACAGTG_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M5_ACAGTG_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24509039
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9211323.7583358531519737Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1087300.043.6847271
GTCGCCG1043800.043.66585544
AGCGTCG1069450.043.6200910
GCGTCGT1072100.043.6026311
CGTCGTG1075150.043.59759512
ATCGGAA1085850.043.567152
GAGCGTC1071900.043.4608849
GTCGTGT1084200.043.36968613
TCGGAAG1092700.043.3581053
TGGTCGC1048000.043.32737742
TCGTGTA1075100.043.32164814
GTGGTCG1056600.043.28428341
CGGAAGA1086300.043.2356644
AGAGCGT1081950.043.2317548
AAGAGCG1082250.043.225697
GGTCGCC1051250.043.2131343
GATCTCG1073650.043.1821934
CGTGTAG1084300.043.1553415
TCGGTGG1081700.043.11919838
CGGTGGT1074550.043.0886839
Produced by FastQC (version 0.11.2)