FastQCFastQC Report
Mon 31 Jul 2017
G120-M11_ATTCCT-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M11_ATTCCT-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025818
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT915410.6526606861717441TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT115150.041.6076944
GATCGGA133700.041.4833761
TCTCGTA109600.041.12829243
TATCTCG108900.041.0318541
CGTCTGA135300.040.91884216
ATCGGAA136900.040.4069022
ACGTCTG137450.040.2593315
ATCTCGT112350.040.04587642
TCGGAAG139900.039.5130923
ACACGTC139950.039.46140713
CGGAAGA141550.038.9844554
CACGTCT146500.037.8472814
GCACACG147400.037.59629411
CACACGT150500.036.92526612
GAACTCC163750.033.98221
TCTGAAC172200.032.27013418
GTCACAT156300.032.03829629
CAGTCAC160900.032.02512727
TCCAGTC173750.031.69942525
CCAGTCA165150.031.44358626
Produced by FastQC (version 0.11.2)