FastQCFastQC Report
Mon 31 Jul 2017
G120-M6_GTGAAA-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M6_GTGAAA-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960962
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT591110.45606954175160763TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80950.041.177551
CTCGTAT72850.040.78029344
AACGATC66200.040.56151238
TCTCGTA67050.040.53772443
ATCGGAA83600.039.670822
CGATCTC68000.039.6495340
CGTCTGA84450.039.44854416
ACGATCT68900.039.16352539
AAACGAT71250.039.1050737
GATCTCG69050.039.07845341
ACACGTC85350.039.0556313
ATCTCGT69700.038.90332842
ACGTCTG86000.038.6836915
GAAACGA80600.038.43884736
TGAAACG81500.038.32905235
AGTCACG83950.037.93644728
GTCACGT83500.037.8509529
CACGTCT89500.037.4167614
TCGGAAG88650.037.415423
CGGAAGA88900.037.270684
Produced by FastQC (version 0.11.2)