FastQCFastQC Report
Mon 31 Jul 2017
G120-M7_GTGGCC-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M7_GTGGCC-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13934259
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT481490.345544029287815TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA65950.041.128381
CTCGTAT63050.039.68647844
TCTCGTA59150.039.48026743
ATCGGAA68800.039.2576522
TATCTCG58250.039.1482141
CGTCTGA70450.038.63726816
ATCTCGT61000.038.0322242
ACACGTC72200.037.69711313
TCGGAAG72050.037.5534973
ACGTCTG73550.037.154915
ACGTGGC68750.037.0602532
CGTGGCC68700.036.95911433
GTCACGT70950.036.733129
AGTCACG71250.036.70200728
CGGAAGA74950.035.902784
CACGTCT76750.035.66297514
TCACGTG76250.033.94450830
GCACACG81800.033.43018711
CACGTGG78050.033.01498431
CACACGT83800.032.73816312
Produced by FastQC (version 0.11.2)