FastQCFastQC Report
Mon 31 Jul 2017
G120-M8_GTTTCG-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M8_GTTTCG-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10626376
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT198270.18658289524104926TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG24550.041.31712733
GATCGGA30950.038.2755741
CTCGTAT26150.038.17368344
ATCGGAA31650.037.497262
TTTCGGA26950.037.22332435
GTTTCGG27350.037.1673234
TCTCGTA24300.036.9202443
CGGAATC23750.036.75851438
TCGGAAT24500.036.530337
CGTCTGA32900.035.9168316
AATCTCG24800.035.37954741
TTCGGAA28150.035.305836
ACACGTC33750.034.87922713
AGTCACG30400.034.2461328
ACGTCTG34600.034.21314215
TCGGAAG34950.034.087813
GTCACGT30250.034.05214329
ATCTCGT27100.033.10716242
CGGAAGA35700.033.0717664
ACGTTTC31400.032.16353232
Produced by FastQC (version 0.11.2)