FastQCFastQC Report
Mon 31 Jul 2017
G120_M6-M11.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M6-M11.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74837567
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA448000.040.8083571
CTCGTAT394250.040.35688844
TCTCGTA371050.039.8121743
ATCGGAA459750.039.632932
CGTCTGA461450.039.46035816
CCGTACG80250.038.93428433
ACACGTC474550.038.50271613
ACGTCTG474450.038.50163315
CGTACGT81700.038.18860234
ATCTCGT387450.038.0489442
TCGGAAG482250.037.757383
CGGAAGA491750.036.87514
TATCTCG224250.036.7724941
CACGTCT504600.036.33625414
ACCGTAC88700.035.175632
CACACGT530950.034.64912412
GCACACG532000.034.57964711
CGTAATC79050.034.52185438
CGTTTCG33500.033.8917333
GTACGTA92700.033.4847235
Produced by FastQC (version 0.11.2)