FastQCFastQC Report
Mon 31 Jul 2017
G120_M6-M11.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M6-M11.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74837567
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2760400.36885218355642163Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG323200.041.57697744
CGTCGTG447450.040.57584412
GATCGGA450750.040.544281
AGCGTCG448800.040.38993510
GCGTCGT450500.040.29628411
TGGTCGC328850.040.17294742
ATCGGAA460100.039.711452
GATCTCG361500.039.5145834
GTGGTCG343400.039.3484941
GGTCGCC340850.039.29432743
CGTGTAG466600.038.96121615
GAGCGTC466200.038.8352979
GTCGTGT469000.038.81379713
TCGTGTA468250.038.74424414
TCGGAAG480500.038.0847473
TCGGTGG386150.037.9036638
ATCTCGG381400.037.89124335
AGAGCGT482600.037.6340688
TCTCGGT379000.037.4926736
AAGAGCG489050.037.1490257
Produced by FastQC (version 0.11.2)