FastQCFastQC Report
Mon 31 Jul 2017
G120_M6M7M8.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M6M7M8.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37521597
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1133310.3020420479437483Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG136900.041.10770444
CGTCGTG183300.040.757412
AGCGTCG183200.040.6833110
GCGTCGT184900.040.42856211
GATCGGA187450.040.2130361
TGGTCGC139300.039.59346842
ATCGGAA191250.039.391922
GATCTCG154950.038.80348634
GAGCGTC193050.038.573399
GTCGTGT194500.038.5339913
TCGTGTA194050.038.45314814
GTGGTCG147600.038.29112241
CGTGTAG196100.038.17440815
GGTCGCC146950.038.10119243
TCGGAAG201850.037.3556333
AGAGCGT201500.037.119568
ATCTCGG165900.036.7197135
AAGAGCG204350.036.5395627
TCGGTGG169800.036.29088238
TCTCGGT165100.036.17807436
Produced by FastQC (version 0.11.2)