FastQCFastQC Report
Mon 31 Jul 2017
G120_M12M13.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M12M13.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113077723
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT103100.029.66722542
TCGTATG108700.029.35128443
TCTCGTA99000.028.91907741
GATCGGA116450.028.6698951
CGTATGC111050.028.60961544
ATCGGAA128900.025.828932
CACCGAT93200.025.07535431
CGTCTGA132750.024.88121216
CGATGTA92700.024.68501734
ACACGTC136350.024.4479413
ACCGATG95450.024.34328332
ACGTCTG148100.022.71641215
ATCTCGT126100.022.70411340
TATCTCG93900.022.3872639
CCGATGT104050.022.22551233
GTCACCG107500.021.90897829
CACGACG26900.021.75788315
ACGACGC26850.021.47248816
TCGGAAG156350.021.2972033
CACGTCT162900.020.69295314
Produced by FastQC (version 0.11.2)