FastQCFastQC Report
Mon 31 Jul 2017
G120_M12M13.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120_M12M13.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113077723
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG86000.037.55021712
GCGTCGT87500.036.7307611
AGCGTCG88350.036.402110
GTCGCCG68950.035.19383644
GATCGGA113100.029.3005731
TGGTCGC84550.028.41345242
GTCGTGT115350.028.30005513
GAGCGTC116900.027.7752279
TCGTGTA120150.027.18768114
GGTCGCC92350.026.56152243
GTGGTCG94550.026.31577141
ATCGGAA126300.026.1690542
CGTGTAG126300.025.75924315
GATCTCG106550.024.96260834
AGAGCGT138250.023.6450088
AAGAGCG145350.022.325517
TCGGAAG151200.021.9028193
ATCTCGG126550.021.48688335
TCGGTGG129300.021.33613438
TCTCGGT129250.020.4252736
Produced by FastQC (version 0.11.2)