FastQCFastQC Report
Sun 2 Apr 2017
G121-M1_ATCACG-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M1_ATCACG-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25889137
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG795040.30709405261365025Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG126250.041.0192812
GCGTCGT126550.040.8003511
AGCGTCG126850.040.5119910
GTCGCCG98750.039.61025244
GATCGGA132100.039.5067981
TGGTCGC100150.038.72688742
TCGTGTA134800.038.6477414
GATCTCG111500.038.43590534
GTCGTGT135850.038.38059613
CGTGTAG136150.038.37763615
ATCGGAA135600.038.3243032
GTGGTCG105300.037.89746541
GAGCGTC136300.037.7513289
ATCTCGG118050.037.01132235
GGTCGCC105500.036.99239343
AGAGCGT140750.036.6658338
TCGGTGG124700.035.81273738
AAGAGCG143800.035.7964327
TCGGAAG146100.035.675133
TCTCGGT121100.035.55212436
Produced by FastQC (version 0.11.2)