Basic Statistics
Measure | Value |
---|---|
Filename | G121-M2_CGATGT-_BC7MKJANXX_L007_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 30110099 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 1317778 | 4.376531608215569 | TruSeq Adapter, Index 2 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 160295 | 0.0 | 43.5351 | 1 |
ATCGGAA | 160340 | 0.0 | 43.340954 | 2 |
TCGGAAG | 162115 | 0.0 | 42.96576 | 3 |
ACACGTC | 163380 | 0.0 | 42.87628 | 13 |
CGGAAGA | 162505 | 0.0 | 42.86713 | 4 |
CGTCTGA | 162795 | 0.0 | 42.84519 | 16 |
ACGTCTG | 163650 | 0.0 | 42.759827 | 15 |
CACGTCT | 164485 | 0.0 | 42.624287 | 14 |
CGTATGC | 160855 | 0.0 | 42.576176 | 44 |
CGATGTA | 156430 | 0.0 | 42.492424 | 34 |
CTCGTAT | 157905 | 0.0 | 42.45229 | 42 |
CACCGAT | 158760 | 0.0 | 42.451466 | 31 |
ACCGATG | 158310 | 0.0 | 42.435917 | 32 |
TATCTCG | 147620 | 0.0 | 42.425987 | 39 |
TCGTATG | 161460 | 0.0 | 42.425728 | 43 |
CCGATGT | 157440 | 0.0 | 42.401596 | 33 |
TCTCGTA | 149125 | 0.0 | 42.39348 | 41 |
GTCACCG | 161880 | 0.0 | 42.26454 | 29 |
CACACGT | 166055 | 0.0 | 42.242558 | 12 |
TCACCGA | 161000 | 0.0 | 42.239902 | 30 |