FastQCFastQC Report
Sun 2 Apr 2017
G121-M2_CGATGT-_BC7MKJANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M2_CGATGT-_BC7MKJANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30110099
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10233053.3985441230199873Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG316930.10525704349228476Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1556100.043.485271
GCGTCGT1552750.043.4679711
CGTCGTG1557600.043.44703312
AGCGTCG1549950.043.43802610
ATCGGAA1554300.043.424422
GTCGCCG1177250.043.40236344
TCGTGTA1562950.043.2247914
GTCGTGT1571700.043.1980213
GAGCGTC1559550.043.178899
TGGTCGC1163400.043.1228342
CGTGTAG1568800.043.0860415
GATCTCG1304250.043.05056434
TCGGAAG1573550.043.031473
AGAGCGT1570400.043.022168
ATCTCGG1323300.042.96246335
GTGGTCG1202500.042.93822541
GGTCGCC1187900.042.93717243
AAGAGCG1565900.042.9140747
CGGAAGA1564650.042.8937644
TCTCGGT1298450.042.77296436