Basic Statistics
Measure | Value |
---|---|
Filename | G121-M2_CGATGT-_BC7MKJANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 30110099 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1023305 | 3.3985441230199873 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG | 31693 | 0.10525704349228476 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 155610 | 0.0 | 43.48527 | 1 |
GCGTCGT | 155275 | 0.0 | 43.46797 | 11 |
CGTCGTG | 155760 | 0.0 | 43.447033 | 12 |
AGCGTCG | 154995 | 0.0 | 43.438026 | 10 |
ATCGGAA | 155430 | 0.0 | 43.42442 | 2 |
GTCGCCG | 117725 | 0.0 | 43.402363 | 44 |
TCGTGTA | 156295 | 0.0 | 43.22479 | 14 |
GTCGTGT | 157170 | 0.0 | 43.19802 | 13 |
GAGCGTC | 155955 | 0.0 | 43.17889 | 9 |
TGGTCGC | 116340 | 0.0 | 43.12283 | 42 |
CGTGTAG | 156880 | 0.0 | 43.08604 | 15 |
GATCTCG | 130425 | 0.0 | 43.050564 | 34 |
TCGGAAG | 157355 | 0.0 | 43.03147 | 3 |
AGAGCGT | 157040 | 0.0 | 43.02216 | 8 |
ATCTCGG | 132330 | 0.0 | 42.962463 | 35 |
GTGGTCG | 120250 | 0.0 | 42.938225 | 41 |
GGTCGCC | 118790 | 0.0 | 42.937172 | 43 |
AAGAGCG | 156590 | 0.0 | 42.914074 | 7 |
CGGAAGA | 156465 | 0.0 | 42.893764 | 4 |
TCTCGGT | 129845 | 0.0 | 42.772964 | 36 |