Basic Statistics
Measure | Value |
---|---|
Filename | G121-M4_TGACCA-_BC7MKJANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 26788213 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 655144 | 2.4456427907303855 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTCGT | 98525 | 0.0 | 43.443466 | 11 |
AGCGTCG | 98480 | 0.0 | 43.39245 | 10 |
CGTCGTG | 98785 | 0.0 | 43.38043 | 12 |
GTCGCCG | 74850 | 0.0 | 43.319866 | 44 |
GATCGGA | 98910 | 0.0 | 43.30478 | 1 |
ATCGGAA | 99000 | 0.0 | 43.249367 | 2 |
TCGTGTA | 99480 | 0.0 | 43.053917 | 14 |
TGGTCGC | 74775 | 0.0 | 43.03086 | 42 |
GTCGTGT | 99945 | 0.0 | 43.027184 | 13 |
GAGCGTC | 99385 | 0.0 | 42.959526 | 9 |
CGTGTAG | 99845 | 0.0 | 42.91856 | 15 |
GATCTCG | 82985 | 0.0 | 42.90795 | 34 |
GTGGTCG | 77345 | 0.0 | 42.899937 | 41 |
AGAGCGT | 100200 | 0.0 | 42.775284 | 8 |
ATCTCGG | 84510 | 0.0 | 42.750324 | 35 |
TCGGAAG | 100385 | 0.0 | 42.737976 | 3 |
GGTCGCC | 75910 | 0.0 | 42.732338 | 43 |
AAGAGCG | 99995 | 0.0 | 42.676384 | 7 |
TCTCGGT | 83435 | 0.0 | 42.560116 | 36 |
CGGAAGA | 100245 | 0.0 | 42.49259 | 4 |