FastQCFastQC Report
Sun 2 Apr 2017
G121-M5_ACAGTG-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M5_ACAGTG-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26983163
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC6305242.3367312423676943TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA764150.043.3321271
ATCGGAA766050.043.068132
CGTATGC769850.042.64880844
TCGTATG771900.042.57074743
TCTCGTA690100.042.5647541
CGTCTGA779100.042.5622816
CTCGTAT740100.042.5593142
ACACGTC784900.042.44103213
GATCTCG689300.042.38221439
TCGGAAG780400.042.3780443
ACGTCTG787350.042.281115
ATCTCGT694000.042.26218440
CGGAAGA784600.042.1272244
CACGTCT795600.041.8839914
CACACGT806500.041.38885512
GCACACG807950.041.27802311
AGTGATC719700.040.14619436
GAACTCC834000.039.49632621
GTGATCT734150.039.477837
GTCACAC832900.039.20890429
Produced by FastQC (version 0.11.2)