FastQCFastQC Report
Sun 2 Apr 2017
G121-M6_GCCAAT-_BC7MKJANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121-M6_GCCAAT-_BC7MKJANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26031400
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8920493.426819149181373TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1074950.043.4914051
ATCGGAA1077050.043.2472762
TCGGAAG1089850.042.81973
CGTCTGA1093800.042.79961816
ACACGTC1097450.042.76987513
ACGTCTG1099100.042.6513715
CGGAAGA1093850.042.6439064
CGCCAAT1057800.042.55953633
CGTATGC1078950.042.47958844
GTCACGC1080900.042.45928229
CACGTCT1106700.042.38837414
TATCTCG994300.042.3856539
ACGCCAA1067950.042.37181532
TCTCGTA1005150.042.30139541
TCGTATG1083950.042.29240443
CTCGTAT1066350.042.26353542
AGTCACG1092900.042.24147828
TCACGCC1084250.042.2218230
CACACGT1116600.042.12117812
ATCTCGT1007700.042.1114340