Basic Statistics
Measure | Value |
---|---|
Filename | G121-M6_GCCAAT-_BC7MKJANXX_L007_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 26031400 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 695927 | 2.673413646596034 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 104735 | 0.0 | 43.43658 | 1 |
CGTCGTG | 104850 | 0.0 | 43.403236 | 12 |
AGCGTCG | 104410 | 0.0 | 43.401413 | 10 |
GCGTCGT | 104620 | 0.0 | 43.38931 | 11 |
ATCGGAA | 104820 | 0.0 | 43.300102 | 2 |
GTCGCCG | 79255 | 0.0 | 43.27439 | 44 |
GTCGTGT | 105825 | 0.0 | 43.155853 | 13 |
TCGTGTA | 105360 | 0.0 | 43.118965 | 14 |
CGTGTAG | 105610 | 0.0 | 43.035645 | 15 |
GAGCGTC | 105175 | 0.0 | 43.01671 | 9 |
TGGTCGC | 79155 | 0.0 | 42.94822 | 42 |
TCGGAAG | 105960 | 0.0 | 42.919216 | 3 |
AGAGCGT | 106120 | 0.0 | 42.847836 | 8 |
GATCTCG | 87285 | 0.0 | 42.833317 | 34 |
GTGGTCG | 82090 | 0.0 | 42.764793 | 41 |
GGTCGCC | 80190 | 0.0 | 42.758766 | 43 |
AAGAGCG | 105770 | 0.0 | 42.736217 | 7 |
ATCTCGG | 89020 | 0.0 | 42.623425 | 35 |
CGGAAGA | 106045 | 0.0 | 42.55078 | 4 |
TCTCGGT | 87540 | 0.0 | 42.54462 | 36 |