FastQCFastQC Report
Sun 2 Apr 2017
G121_M1-M6.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M1-M6.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162581123
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG33612792.0674472767665653Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT5121200.043.3557411
CGTCGTG5135150.043.346412
AGCGTCG5107950.043.3405310
GTCGCCG3893100.043.2496644
GATCGGA5148300.043.23131
ATCGGAA5154100.043.0923842
TCGTGTA5172850.042.96542714
GTCGTGT5197450.042.9525813
GAGCGTC5158800.042.882889
TGGTCGC3883750.042.85128842
CGTGTAG5195100.042.82460815
GATCTCG4310800.042.7386734
GTGGTCG4017750.042.67759741
GGTCGCC3945700.042.63903443
AGAGCGT5212200.042.61438
ATCTCGG4385800.042.55260535
TCGGAAG5231450.042.5303733
AAGAGCG5205750.042.4409187
TCGGTGG4470350.042.3045838
TCTCGGT4326400.042.27734836