Basic Statistics
Measure | Value |
---|---|
Filename | G121_M1-M6.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 162581123 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 3361279 | 2.0674472767665653 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTCGT | 512120 | 0.0 | 43.35574 | 11 |
CGTCGTG | 513515 | 0.0 | 43.3464 | 12 |
AGCGTCG | 510795 | 0.0 | 43.34053 | 10 |
GTCGCCG | 389310 | 0.0 | 43.24966 | 44 |
GATCGGA | 514830 | 0.0 | 43.2313 | 1 |
ATCGGAA | 515410 | 0.0 | 43.092384 | 2 |
TCGTGTA | 517285 | 0.0 | 42.965427 | 14 |
GTCGTGT | 519745 | 0.0 | 42.95258 | 13 |
GAGCGTC | 515880 | 0.0 | 42.88288 | 9 |
TGGTCGC | 388375 | 0.0 | 42.851288 | 42 |
CGTGTAG | 519510 | 0.0 | 42.824608 | 15 |
GATCTCG | 431080 | 0.0 | 42.73867 | 34 |
GTGGTCG | 401775 | 0.0 | 42.677597 | 41 |
GGTCGCC | 394570 | 0.0 | 42.639034 | 43 |
AGAGCGT | 521220 | 0.0 | 42.6143 | 8 |
ATCTCGG | 438580 | 0.0 | 42.552605 | 35 |
TCGGAAG | 523145 | 0.0 | 42.530373 | 3 |
AAGAGCG | 520575 | 0.0 | 42.440918 | 7 |
TCGGTGG | 447035 | 0.0 | 42.30458 | 38 |
TCTCGGT | 432640 | 0.0 | 42.277348 | 36 |