FastQCFastQC Report
Sun 2 Apr 2017
G121_M1M2M3.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M1M2M3.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82778347
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC979260.11829905228718811TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2383400.043.194681
ATCGGAA2391700.042.8918652
CGTATGC2390550.042.51247444
ACACGTC2439300.042.48573313
CGTCTGA2434750.042.47664316
CTCGTAT2343600.042.4118942
TCGTATG2397750.042.3984543
TCTCGTA2207700.042.3832241
TCGGAAG2432150.042.2994233
ACGTCTG2450850.042.2935415
CGATGTA1582250.042.1320834
CGGAAGA2444550.042.0807274
ATCTCGT2225850.041.99025340
CACGTCT2474050.041.9290114
CACCGAT1612900.041.88047431
TATCTCG1503650.041.8086439
ACCGATG1611750.041.76154332
CCGATGT1605200.041.68771433
CACACGT2512450.041.3257812
TCACCGA1648000.041.3117830
Produced by FastQC (version 0.11.2)