FastQCFastQC Report
Sun 2 Apr 2017
G121_M4M5M6.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG121_M4M5M6.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79802776
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC8260501.0351143674500747TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2876800.043.4223371
ATCGGAA2887400.043.150842
CGTCTGA2932950.042.79501316
CGTATGC2890750.042.72838244
ACACGTC2944250.042.72260713
ACGTCTG2950950.042.64057515
TCGGAAG2928900.042.6404533
CTCGTAT2808400.042.59639742
TCGTATG2902550.042.54606243
TCTCGTA2647300.042.53389741
CGGAAGA2937650.042.445574
ATCTCGT2656150.042.30207440
CACGTCT2976250.042.29874814
CGCCAAT1086000.042.1000733
GCACACG3009850.041.80983711
CACACGT3013700.041.80310412
TATCTCG1027700.041.7115639
GTCACGC1120000.041.66381529
ACGCCAA1107250.041.5030332
AATCTCG950300.041.34401739
Produced by FastQC (version 0.11.2)