G121_M4M5M6.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G121_M4M5M6.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79802776
Sequences flagged as poor quality	0
Sequence length	50
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1849463	2.317542186753002	Illumina Single End PCR Primer 1 (100% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCGTCGT	280430	43.388374	11
AGCGTCG	279825	43.351673	10
CGTCGTG	281455	43.340633	12
GATCGGA	281435	43.322933	1
GTCGCCG	213325	43.2978	44
ATCGGAA	281270	43.259274	2
TCGTGTA	282910	43.038742	14
GTCGTGT	284555	42.980473	13
TGGTCGC	213015	42.930035	42
GAGCGTC	282340	42.9287	9
CGTGTAG	283935	42.904293	15
GATCTCG	235920	42.83198	34
GTGGTCG	220140	42.79262	41
AGAGCGT	284500	42.746906	8
TCGGAAG	285215	42.740856	3
GGTCGCC	216270	42.674522	43
ATCTCGG	240050	42.642643	35
AAGAGCG	284250	42.566772	7
TCTCGGT	236900	42.48795	36
CGGAAGA	284685	42.472652	4