FastQCFastQC Report
Tue 28 Feb 2017
G120-M11_ATTCCT-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M11_ATTCCT-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14025818
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG835500.5956871820238934Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG96350.042.47021544
TGGTCGC97350.041.64917842
GGTCGCC99250.041.2508543
GATCGGA135500.041.24521
GATCTCG104700.041.18409334
GTGGTCG101100.040.88770341
CGTCGTG135250.040.8416512
AGCGTCG135000.040.8359510
GCGTCGT135500.040.68526511
CGTGTAG136450.040.43323515
TCGGTGG110100.040.38290838
ATCGGAA138550.040.2755852
TCTCGGT107100.040.1790436
ATCTCGG109150.040.00892635
GAGCGTC137850.040.0077829
GTCGTGT138850.039.9247613
CGGTGGT107850.039.7769539
TCGTGTA139700.039.58735714
CTCGGTG109050.039.46056737
AGAGCGT140550.039.4583478
Produced by FastQC (version 0.11.2)