FastQCFastQC Report
Tue 28 Feb 2017
G120-M12_ATCACG-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M12_ATCACG-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55344692
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT33450.024.25728842
TCGTATG36750.023.57369843
TCTCGTA32150.023.3914741
GATCGGA39600.023.3564471
CGTATGC39750.021.84864244
CACGACG13150.021.58492715
ACGACGC13650.020.95730616
ATCGGAA45750.020.1261582
CGATCTC39150.019.26523638
CGACGCT14250.018.99417917
GATCTCG39250.018.82400339
ACGATCT40900.018.7630737
ACACGTC50000.018.74664113
CGTCTGA50550.018.3702916
CACGATC41100.018.24181436
ATCACGA48200.018.12255734
TCACGAT44700.017.76609635
ATCTCGT45000.016.5653140
ACGTCTG58950.016.23648615
CATCACG55500.015.77898633
Produced by FastQC (version 0.11.2)