FastQCFastQC Report
Tue 28 Feb 2017
G120-M12_ATCACG-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M12_ATCACG-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55344692
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG26750.033.47001612
GCGTCGT26750.032.89454711
AGCGTCG27250.032.5329210
GTCGCCG23000.029.46118544
GATCGGA38150.024.4556661
GTCGTGT40350.022.4607213
TCGTGTA41350.021.97065214
TGGTCGC31300.021.64818842
GAGCGTC42350.021.5046259
ATCGGAA44500.020.9168192
GGTCGCC34850.019.88487843
GTGGTCG35650.019.80891441
CGTGTAG46550.019.56354115
GATCTCG39350.019.34411634
AGAGCGT51500.017.7266148
AAGAGCG55800.016.3621397
TCGGAAG59300.015.7704483
ATCTCGG50200.015.46993435
TCGGTGG51300.015.26684738
CGGTGGT52700.014.35998339
Produced by FastQC (version 0.11.2)