FastQCFastQC Report
Tue 28 Feb 2017
G120-M13_CGATGT-_BC7MKJANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M13_CGATGT-_BC7MKJANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57733031
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72800.032.37535544
TCTCGTA67250.032.14069741
CGATGTA71950.032.0491434
CTCGTAT71550.031.99153742
TCGTATG74350.031.82051843
ACCGATG74750.031.2321732
GATCGGA77450.031.2208921
CACCGAT75700.031.04705831
CGTCTGA82600.029.7310616
GTCACCG79900.029.69827829
CCGATGT78300.029.67564633
TATCTCG73550.029.2381739
ACACGTC84900.029.0008913
ATCGGAA83750.028.9676282
ACGTCTG90350.027.32473215
ATCTCGT81350.026.70505340
TCACCGA90950.026.23269530
TCGGAAG97750.024.9129223
CACGACG13500.024.28531515
CACGTCT103350.023.93015914
Produced by FastQC (version 0.11.2)