FastQCFastQC Report
Tue 28 Feb 2017
G120-M7_GTGGCC-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M7_GTGGCC-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13934259
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG418090.30004465971243965Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG64700.041.34538310
GCGTCGT65100.041.1593711
CGTCGTG65400.040.9032912
GTCGCCG49450.040.79661644
GATCGGA66600.040.568351
ATCGGAA68500.039.4118272
TGGTCGC50450.039.24621642
GAGCGTC68650.038.9984869
CGTGTAG69650.038.5331815
GGTCGCC52000.038.4994543
GTCGTGT70150.038.2901713
GTGGTCG53450.038.19589641
GATCTCG55500.038.1728434
AGAGCGT71050.038.0527048
TCGTGTA70450.038.00221314
ATCTCGG58100.037.0325735
TCGGAAG73200.037.0313073
AAGAGCG73100.036.7168277
TCTCGGT58700.035.79203436
TCGGTGG61300.035.78114738
Produced by FastQC (version 0.11.2)