FastQCFastQC Report
Tue 28 Feb 2017
G120-M8_GTTTCG-_BC7MKJANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG120-M8_GTTTCG-_BC7MKJANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10626376
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG176740.1663219897357293Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG30000.039.3037812
GCGTCGT30300.039.06002411
AGCGTCG30450.038.72330510
GTCGCCG21450.038.66619544
GATCGGA31400.037.5604021
GAGCGTC32650.035.979329
ATCGGAA33400.035.642182
CGTGTAG32900.035.43729415
TGGTCGC23150.034.87613742
TCGTGTA33650.034.7133214
GGTCGCC24150.034.2518543
GATCTCG25700.034.1555534
GTCGTGT34900.033.97426213
GTGGTCG24550.033.78338241
AGAGCGT35600.032.9360968
TCGGAAG36150.032.8087653
ATCTCGG27100.032.63459435
AAGAGCG36400.032.576837
CGGAAGA37100.032.028114
TCTCGGT27300.031.91199936
Produced by FastQC (version 0.11.2)